General considerations mCLING can be also used to study synaptic vesicle recycling in more complex tissue preparations.Of course tissue dissection and treatment must maintain the sample in a viable state to ensure the ongoing of membrane re...
General considerations For homogeneous labeling of the plasma membrane make sure to dissociate cells before plating and use the cells at a confluence rate not above 50% to 70%.Make sure to work with healthy viable cells. mCLING can strongly...
Some antibodies require an additional antigen retrieval (AGR) step for efficient antigen detection. These protocols can be used for PFA fixed tissue sections that are not paraffin embedded. Please refer to the remarks section for IHC on the...
Tissue quality is crucial for the success of immunohistochemicalexperiments. Complete perfusion is important to remove residual IgG containing blood from blood vessels. These IgGs may be bound by cross-reactive secondary reagents and cause...
In fluorescent immunostainings, fluorophore-conjugated secondary antibodiesare used to localize antigen-antibody complexes. Fluorescent detection allows easier Multiplexing, especially for co-localized targets, and has a higher dynamic rang...
Important: Some proteins have special requirements for good detection. Please refer to the remarks sections for IHC-P on the respective data sheet. Tissue preparation For the preparation of paraffin embedded tissues for immunohistochemistry...
This protocol is suitablefor the immunohistochemical analysis of PFA perfused and post-fixed vibratome and cryo tissue-sections. For tissue preparation, please refer to our tissue preparation protocols. Important: For good detection some pr...
Methanol fixation works by denaturing and precipitating proteins, and as such it is a quick method. For most antibodies/proteins, it takes only 5 minutes. This procedure sometimesleads to an unmasking of epitopes. Important: Some proteins h...
Important: Some proteins have special requirements for good detection. Please refer to the remarks sections for ICCon the respective data sheet. Solutions needed PBS: Phosphate buffered saline, pH7.4 Fixation solution : 4% Paraformaldehyd i...
