All steps should be carried out at 4C Solutions needed Homogenization buffer : 320mM sucrose, 4 mMHEPES, pH7.3 PMSF solution : 200mM PMSF in EtOH HEPES buffer: 1M HEPES-KOH, pH7.4 Procedure The protocol is recommended for one brain, but can...
Protein A is a cell wall protein from Staphylococcus aureus and is commonly used for the affinity purification or pull downs of IgG class antibodies. It binds to immunoglobulins through interaction with the IgG heavy chain within the Fc reg...
Materials Maxisorb 96-well plate Specific antibody from speciesA as capture antibody Specific antibody from speciesB or biotinylated antibody from speciesA as detector antibody Goat anti-speciesB IgG peroxidase (HRP) conjugated or Streptavi...
Kit contents (sufficient for 130 extractions of up to 300 g protein) 800-MXK A: SySy MemEx Reagent A, 10 ml 800-MXK B: SySy MemEx Reagent B, 30 ml 800-MXK C: SySy MemEx Reagent C, 10 ml 800-MXK D: SySy MemEx Dye, 300 l Storage: Upon receipt...
The ALFA-tag is a novel, highly versatile epitope tag (core sequence SRLEEELRRRLTE) that can be employed for a wide range of life science applications (see Gtzke 2019 (1)). For immunoprecipitation of ALFA-tagged target proteins, NanoTag Bio...
General considerations UseRNase free molecular biology grade water for all buffers and RNase free tips and tubes. Materials and Solutions needed PBS: Phosphate buffered saline, pH7.4 Protein A or protein G sepharose Ice IPP buffer: Tris-HCl...
General considerations UseRNase-free molecular biology-grade water for all buffers and RNase-free tips and tubes. Materials and solutions needed Total RNA or mRNA preparation kit RNase-free molecular biology-grade water Fragmentation buffer...
Materials and solutions needed RNase-free molecular biology-grade water PBS: Phosphate buffered saline, pH7.4 Protein A or protein G sepharose Ice IPP buffer: Tris-HCl pH 7.4, 150 mM NaCl, 0.1% NP40 Phenol/chloroform RNA-elution buffer: Tri...
NanoTags Selector resins are based on high-affinity single-domain antibody (sdAb) fragments covalently immobilized on 4% cross-linked agarose beads. The innovative, oriented and selective attachment via flexible linkers guarantees optimal a...
